The characterization of the expression and regulation of growth-related genes in the muscles of Chinese perch is of great interest to aquaculturists because of the commercial value of the species.The transcriptome annotation of the skeletal muscles is a crucial step in muscle growth-related gene analysis.In this study, we generated 52 504 230 reads of mRNA sequence data from the fast muscles of the Chinese perch by using Solexa/Illumina RNA-seq.
Twenty-one amino acid transporter genes were annotated by searching protein and gene ontology databases, and postprandial changes in their transcript abundance were assayed after administering a single satiating meal to Chinese perch juveniles (body Alpha-GPC mass, approximately 100 g), following fasting for 1 week.The gut content of the Chinese perch increased significantly after Stock Pots 1 h and remained high for 6 h following the meal and emptied within 48-96 h.Expression of eight amino acid transporter genes was assayed in the fast muscles through quantitative real-time polymerase chain reaction at 0, 1, 3, 6, 12, 24, 48, and 96 h.
Among the genes, five transporter transcripts were markedly up-regulated within 1 h of refeeding, indicating that they may be potential candidate genes involved in the rapid-response signaling system regulating fish myotomal muscle growth.These genes display coordinated regulation favoring the resumption of myogenesis responding to feeding.